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Researchers Establish An effective Analytical Quantification NIR-model For Polysaccharides In Mycelia Of Genus Ganoderma
Date: 2018/01/17 Author: MA Yuhan

Recently, a research team led by Prof. HUANG Qing in Institute of Technical Biology & Agriculture Engineering, Hefei Institutes of Physical Science developed an effective approach combining mid-infrared (mid-IR) and near-infrared (NIR) spectroscopy to realize quantitative assessment of the total polysaccharides in Ganoderma mycelia. The paper entitled Assessment of Polysaccharides from Mycelia of genus Ganoderma by Mid-Infrared and Near-Infrared Spectroscopy was published in Scientific Reports.

Genus Ganoderma has been adopted as traditional Chinese herbal medicine in China since ancient times. Ganoderma polysaccharides extracted from mycelia and spores are used as prescription drugs in China by many pharmaceutical factories and companies.

Conventionally, polysaccharides can be examined through biochemical approaches such as the phenol-sulphuric acid method or anthrone-sulphuric acid method. However, these methods normally require deleterious substances such as phenol, anthrone, and sulfuric acid. It is therefore necessary to develop more convenient and efficient approaches to obtain quantitative assessment of polysaccharides in Ganoderma mycelia.

For this purpose, HUANG’s team employed infrared spectroscopy to evaluate polysaccharides in mycelia of different Ganoderma strains produced in the fermentation process.

Based on mid-IR spectroscopy, they measured and analyzed different Ganoderma samples including high-content-polysaccharide (HCP) and low-content-polysaccharide (LCP) G.

They obtained lingzhi strains in different extraction processes, and identified the most relevant mid-IR spectral bands relevant to the polysaccharide evaluation.

Then, based on NIR spectroscopy, they not only identified the most relevant NIR characteristic bands through correlation coefficients method, but also achieved an effective and accurate quantification model for the total polysaccharides in Ganoderma mycelia due to application of different algorithms such as moving window partial least-squares (mwPLS) and interval PLS (iPLS).

As a result, they demonstrated that the NIR-model could be applied to quality control of Ganoderma polysaccharides acquired from industrial production.

This work was supported by the National Nature Science Foundation of China, and the Strategic Priority Research Program of the Chinese Academy of Sciences.

Schematic illustration of quantitative assessment for Ganoderma lingzhi mycelia polysaccharides (Image by MA Yuhan)

 

Contact:
HUANG Qing
Institute of Technical Biology & Agriculture Engineering (http://www.itb.cas.cn/)
Tel: 86-551-65595261
Email: huangq@ipp.ac.cn

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