Plasma, known as the fourth state of matter, is mainly composed of ions, electrons and ionization of neutral particles, which can be divided into high temperature and non-thermal plasma. In recent years, studies and applications of non-thermal plasma in medicine is widely reported, especially the basic research of non-thermal plasma in cancer treatment. Although non-thermal plasma has been predicted as a potential method in cancer therapy, profiling of non-thermal plasma-induced gene expression and regulation of signal pathways still remains unknown.
Lately, a study team from Radiation Division, center of medical physics and technology, Hefei Institutes of Physical Sciences, Chinese Academy of Science (CAS) reports new progress in study of non-thermal plasma-induced gene expression profiling and cell signaling pathways identification performing with non-small-cell lung cancer A549 cells.
The study team, led by Prof. HAN Wei, conducted a systematic research of gene expression profiles in A549 lung cancer cell, which found that more than 1200 genes were significantly modulated by NTP exposures with the transcriptome microarray chip analysis and the conventional real-time fluorescent quantitative PCR that encodes molecules to regulate cell apoptosis process, cellular response to stress, cell cycle, DNA damage response and so on.
Furthermore, signaling and network analysis revealed that certain significantly regulated genes were involved in multiple signaling pathways, including p53, MAPK, PI3K/Akt, JNK and TNF signaling pathways. And, western blotting analysis indicated that NTP exposures induced cell apoptosis and cell cycle which would be of help to cell repair.
These findings lay foundation for further studies to clarify the roles of multiple pathways in plasma-induced biological processes.
These results have been published in BMC Genomics with the title Non-thermal plasma treatment altered gene expression profiling in non-small-cell lung cancer A549 cells.
This research was supported by Hundred-Talent Program of the Chinese academy of sciences and Presidential Foundation of Hefei institutes of Physical Science.
The details of selected pathways after NTP exposure. The nodes represented genes in each pathway, with red and green colors of nodes indicating up- or down-regulated genes. The green line and yellow line represented activation and expression relationship in gene interaction enrichment. (Image by HOU Jue and MA Jie)
Prof. HAN Wei, Ph.D Principal Investigator
Center for Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences
Hefei, Anhui 230031, China
Tel: +86 551 65595100