Graphene-based Nanozyme Helps to Detect L-cysteine in Serum

Graphene-based materials can be obtained using various reducing agents, many of which are dangerous and toxic chemicals. The obtained graphene-based materials are prone to aggregation, which limits their practical applications. Therefore, looking for other synthetic methods to produce high-quality graphene-based materials, including the use of green natural products, has become a new trend.

Recently, the research group of Prof. HUANG Qing from the Institute of Intelligent Machines, Hefei Institutes of Physical Science(HFIPS), successfully prepared graphene-based nanozymes through a simple and green preparation method, and verified that it can be used to detect L-cysteine in serum.

In this research, researchers used Ganoderma lucidum extract polysaccharides as reducing agents and stabilizers to prepare graphene-based materials under mild hydrothermal conditions. The polysaccharides in Ganoderma lucidum are rich in types and have many excellent biochemical properties. It is proved that Ganoderma polysaccharide can fully reduce graphene oxide, and the existence of Ganoderma polysaccharide can improve the stability and dispersion of graphene-based materials in water.

They further studied the catalytic process of the as-synthesized nanozyme, which indeed showed good catalytic activity. Based on this activity, they developed a colorimetric method for detection of L-cysteine in serum, an essential amino acid which is involved in protein synthesis, post-translational modification and metabolism.

"Disorder of L-cysteine is associated with severe neurotoxicity and cardiovascular disease,” said Prof. Huang, who has been focusing on the research of Ganoderma lucidum, a genus usually growing on decaying hardwood trees, for decades, “L-cysteine was successfully detected in serum with high sensitivity and selectivity in nanozyme method, suggesting its potential application as an effective nanozyme for biological systems.”

The research work was funded by the National Natural Science Foundation of China.

Link to the paper: A green and facile approach to a graphene-based peroxidase-like nanozyme and its application in sensitive colorimetric detection of L-cysteine

Figure 1. Schematic diagram of the preparation of graphene-based nanozymes and the detection of L-cysteine (Image by LIU Chao)

Figure 2. a-b: determination of L-cysteine; c-d: specific detection of cysteine. (Image by Liu Chao)