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Amplification-Free CRISPR Enables Relative Quantification of Nucleic Acids

Researchers led by Prof. Zhu Ling from Hefei Institutes of Physical Science, Chinese Academy of Sciences, have developed a digital Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) hydrogel fluorescence-enhancing microsphere sensing system (CrisprDEM) that enables amplification-free relative quantification of nucleic acids.